目的 研究金钗石斛药材中的菲类抗肿瘤活性成分。方法 利用各种色谱技术提取分离纯化该药材提取物中的天然产物,并经过波谱方法鉴定化合物结构。采用四甲基偶氮唑蓝(MTT)法、MCF-7乳腺癌细胞, 将单体化合物进行体外抗肿瘤活性研究。结果 从金钗石斛的乙酸乙酯部位分离得到9个化合物,鉴定出菲类化合物4个, 分别为densiflorol B(1)、cypripedin(2)、moscatin(3)、2, 4, 8-三甲氧基菲-3, 7-二醇(4); 香豆素类化合物3个: 香豆素(5)、走马芹内酯(6)、异茴芹内酯(7); 黄酮类化合物1个: 柚皮素(8); 酚类化合物1个:(E)-4-(2-甲氧基乙烯基)苯-1,2-二醇(9)。化合物1~3具有良好的抗肿瘤活性, 它们的IC₅₀值分别为2.99, 5.01和72.68 μmol·L^-1。结论 化合物6, 7, 9为首次从兰科植物中分离得到, 化合物1,2,4,5,8为首次从该植物中分离得到。化合物1~3可作为抗肿瘤活性候选药物。

OBJECTIVE To study the antitumor phenanthrene constituents in Dendrobium nobile. METHODS A variety of chromatographic techniques were applied to extract, isolate and purify natural products from extract of Dendrobium nobile, their structures were identified on the basis of spectral data. The antitumor activity of these monomers in MCF-7 breast cancer cells was studied by MTT assay in vitro. RESULTS Nine compounds were isolated from ethyl acetate part in Dendrobium nobile, there were four phenanthrene compounds, which were densiflorol B(1), cypripedin(2), moscatin(3) and 2,4,8-trimethoxy phenanthrene-3,7-diol(4); three coumarin compounds: coumarin(5), moellendorffiline(6) and isopimpinellin(7); one flavanoid: naringenin(8); one phenol: (E)-4-(2-methoxyvinyl) benzene-1,2-diol(9). Compounds 1-3 had good antitumor activities, their IC₅₀ values were 2.99, 5.01 and 72.68 μmol·L^-1 respectively. CONCLUSION The compounds 6, 7, 9 are isolated from the Orchidaceae family for the first time, compounds 1, 2, 4, 5, 8 are isolated from this plant for the first time. Compounds 1-3 can be used as candidates for anti-tumor drugs.